解剖学和形态学
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听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
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神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
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药理学和药剂学
生理学
基本医疗保健
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热带医学
泌尿学和肾脏学
病毒学
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健康政策和服务
心理学,临床
abstract::Aromatic stacking and hydrogen bonding between nucleobases are two of the key interactions responsible for stabilization of DNA double-helical structures. The present work aims at defining the specific contributions of these interactions to the stability of individual base pairs in DNA. The two DNA double helices inve...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20811
更新日期:2007-10-05 00:00:00
abstract::DNA sequences containing four types of analog nucleosides are described. All four are pyridine derivatives constructed as C-nucleosides so that they mimic the pyrimidine derivatives 2'-deoxyuridine, thymidine or 2'-deoxycytidine, but in all cases the analogs lack the corresponding O2-carbonyls that in duplex DNA are l...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20817
更新日期:2007-10-05 00:00:00
abstract::Since the advent of single particle/molecule microscopies, researchers have applied these techniques to understanding the fluid membranes of cells. By observing diffusion of membrane proteins and lipids in live cell membranes of eukaryotic cells, it has been found that membranes contain a mosaic of fluid compartments....
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20801
更新日期:2007-10-05 00:00:00
abstract::This article measures the rates of folding and unfolding of the collagen-like peptide (Pro-Hyp-Gly)(10) over overlapping concentration and temperature ranges. The data allow calculation of the orders of the folding and the unfolding reactions, the effective Arrhenius activation energies, and numerical solution of the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20787
更新日期:2007-09-01 00:00:00
abstract::An unusual class of nanoscopic, ring-shaped, single-walled biopolymers arises when alphabeta-tubulin is mixed with certain small peptides obtained from various marine organisms and cyanobacteria. The single-ring structures, whose mean molecular weight depends on the specific peptide added to the reaction mixture, usua...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20752
更新日期:2007-08-05 00:00:00
abstract::An increasing number of RNAs are being discovered that perform their functions by undergoing large changes in conformation in response to a variety of cellular signals, including recognition of proteins and small molecular targets, changes in temperature, and RNA synthesis itself. The measurement of NMR residual dipol...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20765
更新日期:2007-08-05 00:00:00
abstract::RNA and DNA molecules experience motions on a wide range of time scales, ranging from rapid localized motions to much slower collective motions of entire helical domains. The many functions of RNA in biology very often require this molecule to change its conformation in response to biological signals in the form of sm...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20650
更新日期:2007-08-05 00:00:00
abstract::A de novo polypeptide GH(6)[(GA)(3)GY(GA)(3)GE](8)GAH(6) (YE8) was designed and genetically engineered to form antiparallel beta-strands of GAGAGA repeats. Modulation of pH enables control of solubility, folding, and aggregation of YE8 by control of the overall polypeptide charge, a consequence of the protonation or d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20730
更新日期:2007-07-01 00:00:00
abstract::The equilibrium structural ensemble of a 20-residue polyglutamic acid peptide (E(20)) was studied with FRET, circular dichroism, and molecular dynamics (MD) simulations. A FRET donor, o-aminobenzamide, and acceptor, 3-nitrotyrosine, were introduced at the N- and C-termini, respectively. Circular dichroism, steady stat...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20719
更新日期:2007-06-15 00:00:00
abstract::A comparison of amino acid sequences of yeast Rer2p and Srt1p Z-prenyltransferases shows that the spatial organization of their substrate tunnels agrees with that determined by X-ray for the E. coli undecaprenyl diphosphate synthase (UPPs). The observed trend in the maxima of product length distribution shifted from C...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20715
更新日期:2007-06-05 00:00:00
abstract::Prolonged exposure (>90 days) of bovine beta-lactoglobulin (BLG) to subdenaturing concentrations of either urea or potassium thiocyanate resulted in the formation of ordered polymers in the form of fibrils. The fibrils obtained with each chaotrope showed major differences in morphology, surface properties, thiol acces...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20704
更新日期:2007-05-01 00:00:00
abstract::The self-cleaving hepatitis delta virus (HDV) ribozyme is essential for the replication of HDV, a liver disease causing pathogen in humans. The catalytically critical nucleotide C75 of the ribozyme is buttressed by a trefoil turn pivoting around an extruded G76. In all available crystal structures, the conformation of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20693
更新日期:2007-04-05 00:00:00
abstract::To investigate the mechanism leading to the reduction in tensile strength of permanent waved human hair, the structure of cross-sections at various depths of permanent waved white human hair was directly analyzed without isolating the cuticle and cortex, using Raman spectroscopy. The beta-sheet and/or random coil cont...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20646
更新日期:2007-02-15 00:00:00
abstract::The pressure-induced structural changes of a de novo designed four-helix bundle protein, (alpha-l-alpha)(2), in aqueous solution have been investigated by FTIR spectroscopy. Changes in the amide I' band intensity show that pressure induces disruption of tertiary interactions and stabilizes the solvated alpha-helical f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20628
更新日期:2007-02-05 00:00:00
abstract::In recent years, a revolution has occurred in the basic sciences, which exploits novel single molecule detection and manipulation tools to track and analyze biopolymers in unprecedented detail. A recent Gordon Research Conference style meeting, hosted by the University of Michigan, highlighted current status and futur...
journal_title:Biopolymers
pub_type:
doi:10.1002/bip.20621
更新日期:2007-02-05 00:00:00
abstract::TC5b is a 20 residue polypeptide notable for its compact tertiary structure, a rarity for a short peptide. This structure is due to the "Trp-cage" motif, an association of aromatic, Pro, and Gly residues. The structure of TC5b has been fully characterized by NMR and electronic circular dichroism (ECD) studies, but has...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20709
更新日期:2007-01-01 00:00:00
abstract::The use of alpha,alpha-disubstituted amino acids represents a valuable strategy to exercise conformational control in peptides. Incorporation of the nonstereogenic alpha-aminoisobutyryl-glycyl (Aib-Gly) dipeptidyl sequence into i+1 and i+2 positions of an acyclic peptide sequence, originally designed and investigated ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20738
更新日期:2007-01-01 00:00:00
abstract::Although the structural analysis of membrane proteins is advancing, an understanding of the basic principles that underlie their folding and assembly remains limited because of the high insolubility intrinsic to these molecules and concomitant challenges in obtaining crystals. Fortunately, from an experimental standpo...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20668
更新日期:2007-01-01 00:00:00
abstract::Regulatory, receptor-binding peptides are considered as the agents of choice for diagnostic imaging and therapy of cancers, because their receptors are overexpressed in various human cancer cells. It has been recently indicated that there is a putative role of NPY in breast tumors. The expression of the two best-inves...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20666
更新日期:2007-01-01 00:00:00
abstract::Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20638
更新日期:2007-01-01 00:00:00
abstract::Successful intracellular delivery of various bioactive molecules has been reported using cell-permeating peptides (CPPs) as delivery vectors. To determine the effects of CPPs on the cellular uptake of immunoglobulin Fab fragment, conjugates of a radio-iodinated Fab fragment with CPPs (CPP-(125)I-Fab) derived from HIV-...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20689
更新日期:2007-01-01 00:00:00
abstract::Peptides based on 2-amino-2,3-dihydro-1H-cyclopenta[b]anthracene-2-carboxylic acid (antAib), a fluorescent, achiral, alpha-amino acid belonging to the class of C(i) (alpha)-->C(i) (alpha) cyclized, C(alpha,alpha)-disubstituted glycines, combined with L-Ala, up to the hexamer level, were synthesized by solution methods...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20841
更新日期:2007-01-01 00:00:00
abstract::The design of modified nucleic acid aptamers is improved by considering thermodynamics and kinetics of their association/dissociation processes. Locked Nucleic Acids (LNA) is a promising class of nucleic acid analogs. In this work the thermodynamic and kinetic properties of a LNA quadruplex formed by the TGGGT sequenc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20591
更新日期:2006-12-15 00:00:00
abstract::Increasing attention is being paid to the role of selenium, both as an essential component required for the activity of many enzymes and in the context of selenium-based pharmaceutical agents. A wide range of therapeutics that include selenium are on the market and under development, such as antihypertensive, anticanc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20592
更新日期:2006-12-15 00:00:00
abstract::Human hair as alpha-keratin fiber exhibits a complex morphology, which for the context of this investigation is considered as a filament/matrix-composite, comprising the intermediate filaments (IF) and a variety of amorphous protein components as matrix. Differential scanning calorimetry (DSC) under aqueous conditions...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20599
更新日期:2006-12-15 00:00:00
abstract::Xylanase XYNII from Trichoderma longibrachiatum is a small protein of the molecular weight 21 kDa, belonging to the family 11 of glycosyl hydrolases, which catalyses hydrolysis of xylan. This article reports thermal stability study of xylanase XYN II conformation in the temperature range 15-65 degrees C by the small a...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20605
更新日期:2006-12-15 00:00:00
abstract::The development of differential scanning calorimetry has resulted in an increased interest in studies of the unfolding process in proteins with the aim of identifying domains and interactions with ligands or other proteins. Several of these studies were done with actin and showed that the thermal unfolding of F-actin ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20567
更新日期:2006-11-01 00:00:00
abstract::Chiral interaction of helical peptide with chiral molecule, and concomitant induction in its helix sense have been demonstrated in optically inactive nonapeptide (1) possessing Gly at its N-terminus: H-Gly-(Delta(Z)Phe-Aib)(4)-OCH(3) (1: Delta(Z)Phe = Z-dehydrophenylalanine; Aib = alpha-aminoisobutyric acid). Spectros...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20557
更新日期:2006-11-01 00:00:00
abstract::The 32-residue leucine zipper subsequence, called here Jun-lz, associates in benign media to form a parallel two-stranded coiled coil. Studies are reported of its thermal unfolding/folding transition by circular dichroism (CD) on samples of natural isotopic abundance and by both equilibrium and spin inversion transfer...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20555
更新日期:2006-10-15 00:00:00
abstract::It was shown earlier, from database analysis, model building studies, and molecular dynamics simulations that formation of cross-strand bifurcated or Extra Watson-Crick hydrogen (EWC) bonds between successive base pairs may lead to extra rigidity to DNA double helices of certain sequences. The strengths of these hydro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20542
更新日期:2006-10-15 00:00:00
abstract::Alpha-synuclein (alpha-Syn) has been identified as a component of intracellular fibrillar deposits in Parkinson's disease. Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of alpha-Syn protein have an important role in causing the dise...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20550
更新日期:2006-10-15 00:00:00
abstract::The visual pigment rhodopsin has been extensively studied for the kinetics of its photointermediates by various spectroscopic methods. Unlike such archaeal retinal proteins as bacteriorhodopsin, visual rhodopsin does not thermally recover its dark state after photoexcitation, which precludes repeated excitation of a s...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20540
更新日期:2006-10-05 00:00:00
abstract::An electron paramagnetic resonance (EPR) study was performed for potato and wheat starch containing Cu2+ ions as a paramagnetic probe. Distribution of water in the starch granules as well as the interactions between the copper and starch matrix of different crystalline structures were determined. EPR spectra of the na...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20504
更新日期:2006-08-15 00:00:00
abstract::Raman spectra of normal hair shafts and hair shafts from patients exhibiting trichothiodystrophy (TTD) were obtained using line focus laser illumination. Because hair from TTD patients has a significant decrease in the content of the sulfur-containing amino acids in comparison to normal hair, the 550-500 cm(-1) disulf...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20515
更新日期:2006-08-15 00:00:00
abstract::We present a novel infrared method to investigate the functionality of a protein monolayer tethered to a metal substrate. The approach employs Surface Enhanced Infrared Absorption Spectroscopy (SEIRAS), which renders high surface sensitivity by enhancing the signal of the adsorbed protein by up to approximately 2 orde...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20501
更新日期:2006-07-01 00:00:00
abstract::Porins from outer membrane of Gram-negative bacteria have a highly stable structure. Our previous studies on porin from Paracoccus denitrificans showed that the outer membrane protein porin is extremely stable toward heat, pH, and chemical denaturants. The major question we have addressed in this paper is whether the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20422
更新日期:2006-07-01 00:00:00
abstract::A simple solid-state NMR method was used to study the structure of (13)C- and (15)N-enriched silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. Carbon-13 and (15)N spectra from alanine- or glycine-labeled oriented dragline silks were acquired with the fiber axis aligned parallel o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20471
更新日期:2006-06-05 00:00:00
abstract::Several new biocompatible and degradable materials were prepared by chemical modification of sodium hyaluronate. The method of activation of hyaluronate by cyanogen bromide was used and subsequent reaction with nucleophile led to the formation of carbamate. This modification of hydroxyl groups of glycosaminoglycans pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20461
更新日期:2006-05-01 00:00:00
abstract::The RNA recognition motif (RRM) is one of the most common RNA binding domains. We have investigated the contribution of three highly conserved aromatic amino acids to RNA binding by the N-terminal RRM of the U1A protein. Recently, we synthesized a modified base (A-4CPh) in which a phenyl group is tethered to adenine u...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20408
更新日期:2006-03-01 00:00:00
abstract::The presence of non-native kinetic traps in the free energy landscape of a protein may significantly lengthen the overall folding time so that the folding process becomes unreliable. We use a computational model alpha-helical hairpin peptide to calculate structural free energy landscapes and relate them to the kinetic...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20388
更新日期:2006-02-15 00:00:00